Crystal dehydration as a new method in MX

Venue

December, 04, 2013 / Room 3303 (Aquarium)

Time

10:00 – 18:00

Topics
Crystal dehydration, phase transition, improvement ofdiffraction power, crystal freezing,room temperature data collection

Abstract:
Synchrotron-based Macromolecular Crystallography is the most important method to investigate three-dimensional structures of biologically and bio-medically relevant macromolecules at atomic resolution. While in recent years much development effort has gone into investigating the procedures of growing crystals of macromolecules, relatively little is known about post-crystallization treatment of crystals. One of the approaches to influence the diffraction power of macromolecular crystals before data collection is to employ controlled dehydration as a means to improve the crystalline order. At the HZB-MX beamlines the dehydration device HC1c has been in operation for more than two years now. In the workshop, the approaches and successes that can be achieved using such a device will be discussed. 

Confirmed speakers

  • Juan Sanchez-Weatherby (Diamond Light Source)
  • Martin Bommer (HU-Berlin)
  • Matthew Bowler (EMBL Grenoble)
  • Ramona Duman (Diamond Light Source)
  • Lina Malinauskaite (Aarhus University)
  • Stephen Marino (Max-Delbrück-Zentrum Berlin)
  • Stefan Klingl (Universität Erlangen)
  • Manfred S. Weiss (HZB)
  • Ralf Siebrecht (Maatel)