MX 14.3



"The Macromolecular Crystallography (MX) group at the Helmholtz-Zentrum Berlin (HZB) is operating three state-of-the-art synchrotron beamlines for MX at BESSY II in Berlin (Heinemann et al., 2003; Mueller et al., 2012, 2015). The radiation source for all three beamlines BL14.1-3 is a superconducting 7T-wavelength shifter. Currently, the three beam lines are the most productive stations for MX in Germany, with about 250 PDB depositions per year and over 1500 PDB depositions in total (Status 10/2015). BL14.1 and BL14.2 are energy tuneable in the range 5.5-15.5 keV, while beam line BL14.3 is a fixed-energy side station operated at 13.8 keV. The HZB-MX beamlines are in regular user operation providing close to 200 beam days per year and about 600 user shifts to approximately 100 research groups across Europe. Additional user facilities include office space adjacent to the beam lines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources."

For more details and current status contact the beamline scientist(s).

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Beamline data
Segment L14
Location (Pillar) 15.2
Source 7T-WLS-2 (Wavelength Shifter)
Monochromator KMC-3
Energy range 13.87 keV
Energy resolution 5 eV
Flux 0.4·1011 (Photons/s/100mA)
Polarisation horizontal
Divergence horizontal 2.5 mrad
Divergence vertical 2.5 mrad
Focus size (hor. x vert.) 180x110 μm
Fixed endstation MX-14-3
Flexible endstation(s) no
User endstation not possible
Distance Focus/last valve 300 mm
Height Focus/floor level 1400 mm
Beam availability 24h/d
Phone +49 30 8062 13432

BL14.3 is a fixed energy beamline for macromolecular crystallography. This beamline has special equipment to perform X-ray diffraction experiments under controlled variation of crystal hydration.

BL14.3 supports the following experimental techniques:

  • De novo structure solution using SAD
  • Crystal screening
  • High resolution diffraction experiments
  • Controlled crystal dehydration using the HC1c-device