MX-14-1

Macromolecular Crystallography 14.1

Macromolecular Crystallography station of BL14.1

"The Macromolecular Crystallography (MX) group at the Helmholtz-Zentrum Berlin (HZB) is operating three state-of-the-art synchrotron beamlines for MX at BESSY II in Berlin (Heinemann et al., 2003; Mueller et al., 2012, 2015). The radiation source for all three beamlines BL14.1-3 is a superconducting 7T-wavelength shifter. Currently, the three beam lines are the most productive stations for MX in Germany, with about 250 PDB depositions per year and over 1500 PDB depositions in total (Status 10/2015). BL14.1 and BL14.2 are energy tuneable in the range 5.5-15.5 keV, while beam line BL14.3 is a fixed-energy side station operated at 13.8 keV. The HZB-MX beamlines are in regular user operation providing close to 200 beam days per year and about 600 user shifts to approximately 100 research groups across Europe. Additional user facilities include office space adjacent to the beam lines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources." http://dx.doi.org/10.17815/jlsrf-2-64

Experimental station BL14.1

Experimental station BL14.1

List of publications
Station data
Temperature range 90 - 293 K
Pressure Range For details contact the station manager.
Detector • X-fluorescence detector: Roentec X-Flash
• Pixel-detector: Pilatus 6M
Manipulators CATS sample changer
Sample holder compatibility For details contact the station manager.
Beamline(s)

MX 14.1

Goniometer Microdiffractometer MD2 with Minikappa goniometer MK3

Methods

Crystallography (biological macromolecules)

The experimental station is installed at the tuneable energy beamline for macromolecular crystallography. This station has a high level of automation and can perform X-ray diffraction experiments of very small crystals up to 15 micrometers.

The station supports the following experimental techniques:

  • De novo structure solution using MAD, SAD 
  • High throuput screening
  • Long wavelength phasing
  • UV radiation damage phasing