HZB Foresight-Workshop Tender X-rays in MX

11th December 2015, Berlin-Adlershof

The workshop is part of the series of foresight workshops organized by the HZB. The central theme of the workshop is the use of tender X-rays in the field of macromolecular crystallography (MX). While typical MX experiments are conducted at wavelengths of around 1.0 Å (energy 12.4 keV), there is an ever growing tendency to also consider wavelengths in the tender X-ray region (1.5 – 3.0 A and beyond) for MX applications. Examples are the possibility to determine the structures of native proteins by sulphur anomalous scattering (native SAD), the unambiguous discrimination from protein-bound solvent ions from water molecules, the determination of oxidation states of relevant metal ions in protein structures, the investigation of solvent and substrate channels using Xenon mapping, etc. In the workshop, some of these modern developments will be highlighted and a case will be made for the increased use of tender X-rays for MX applications.

Program:

9: 00 - 9:10Uwe Mueller, Manfred WeissWelcome
9:10 - 9:40Manfred Weiss
HZB
On the Use of Tender X-rays in Macromolecular Crystallography
9:40 - 10:25Wayne Hendrickson
Columbia University
Optimization of Anomalous Diffraction Analyses for
Native Biomolecules
10:25 - 10:55Oliver Einsle
Universität Freiburg
Analysis of Bioinorganic Metal Sites by Spatially Refined
Anomalous Dispersion
10:55 - 11:20Coffee Break
11:20 - 11:40Andreas Förster
DECTRIS
Pushing the limits of experimental phasing with new HPC detectors
11:40 - 12:25James Holton
Berkeley
SHSSS! The dominant source of error anomalous
difference measurements
12:25 - 12:45Michele Cianci
EMBL Hamburg
Data collection with a tailored X-ray beam size at 2.69 Å
wavelength (4.6 keV): Sulfur SAD phasing of Cdc23Nterm
12:45 - 13:45Lunch Break
13:45 - 14:15Meitian Wang
SLS at PSI
Native-SAD Phasing in de novo Macromolecular Structure
Determination
14:15 - 14:45Leo Chavas
SOLEIL
Structure Determination of Naturally Occurring Crystals
via Soft X-rays
14:45 - 15:15Armin Wagner
Diamond
The long-wavelength MX beamline at Diamond Light Source
15:15 - 15:45Markus Wahl
FUB
Structure validation and phasing using tender X-rays
15:45 - 16:15Coffee Break
16:15 - 16:45Oliver Daumke
MDC
Structural studies of dynamin superfamily GTPases
16:45 - 17:05Manuela Gorgel
LMU Munich
Against the Odds? De-novo Structure Determination of
a Single Cysteine Bridge Protein
17:05 - 17:25Karol Nass
MPI for Medical Research
Towards protein crystal structure determination using
sulfur phasing at X-ray free electron lasers
17:30 - 19:00Roundtable discussion
19:00 -20:00Light Dinner for all participants
20:30 - 22:30Speakers Dinner

Meeting Venue

Helmholtz-Zentrum Berlin
Albert-Einstein-Str. 15
12489 Berlin-Adlershof
Germany