Structure of the month - September 2010

Wahl_sep2010_fig3

Figure 1. Overview of the CASK4M-Mg2+-AMPPNP crystal structure. A) Orthogonal ribbon plot of CASK4M CaM-K kinase domain with landmark functional elements colored. Gly-rich loop (GR-loop) - brown, catalytic loop (C-loop) - yellow; DFG motif of the Mg2+-binding loop - orange; activation segment - green; C-terminal Ca2+-CaM-binding element - red. AMPPNP and residues Asn146 and Asp162, which coordinate the Mn2+ ion, are shown as sticks and colored by atom type. Carbon - beige; oxygen - red; nitrogen - blue; phosphorus - pink. B) Structure of CASK CaM-K kinase domain in complex with AMPPNP (sticks) lacking a divalent metal ion [β and γ phosphates disordered; pdb ID 3C0H; (21)] in the same orientation as the CASK4M CaM-K kinase domain in A. Cys146 is shown as sticks. Functional elements are colored as in A. C) Structure of CaMKII kinase domain [pdb ID 2BDW; (28)] in the same orientation as the CASK4M CaM-K kinase domain in A. Asn140 and Asp156, whose equivalents in CASK4M2+ ion, are shown as sticks. Functional elements are colored as in A.


Table 1. Diffraction data and refinement statistics.