Program
Sunday, 9th October 2022HZB, Lecture Hall, Albert-Einstein-Str. 15, 12489 Berlin |
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| 16:00 | Arrvial and registration, setting up of posters |
| 18:00 | Integrative Stuctural Biology in bacterial transcription - Markus Wahl (FU Berlin & HZB) |
| 19:00 | Mixer and poster session |
Monday, 10th October 2022HZB, Lecture Hall, Albert-Einstein-Str. 15, 12489 Berlin |
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| 09:00 | Greetings - Bernd Rech (HZB) |
| 09:15 | Greetings - Manfred Weiss (HZB) |
| 9:30 | Structure determination by MX, an Overview - Manfred Weiss (HZB) |
| 10:30 | Coffee Break |
| 11:00 | Sample preparation for data collection - Uwe Mueller (HZB) |
| 12:00 | Lunch |
| 13:00 | Everything I want to know about my protein (biochemistry, biophysics, bioinformatics) - Bernhard Loll (FU Berlin) |
| 14:00 | Protein crystallization - Yvette Roske (MDC) |
| 15:00 | Coffee Break |
| 15:30 | Construct design - Gert Weber (HZB) |
| 16:30 | Crosslink Mass Spectrometry – Juri Rappsilber (FU Berlin) |
| 17:30 | Serial Synchrotron Crystallography - Thomas Schneider (EMBL Hamburg) |
| 18:30 | Discussion |
| 19:00 | Dinner and poster session |
Tuesday, 11th October 2022HZB, Room "Kino", Magnusstr. 2, 12489 Berlin |
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| 9:00 | Data collection: The last experiment - Ana Gonzalez (MAX IV) |
| 10:00 | Radiation damage - Elspeth Garman (University of Oxford) |
| 11:00 | Coffee Break |
| 11:30 | Crystal dehydration and RT data collection - Gert Weber (HZB) |
| 12:30 | Lunch |
| 13:30 | Preparation of practical part , Data quality issues |
| 13:45 |
(there will be divides into 3 groups (A/B, B/A, C/D) Practical Part A -> Tour of the HZB MX Beamlines incl. Optics Hutch - Uwe Mueller Followed by Data Collection on BL 14.1 - Frank Lennartz & Tom Crosskey Practical Part B -> Crystal Soaking and fishing - Tatjana Barthel, Jan Wollenhaupt, Laila Benz Pracitcal Part C -> Gene and construct optimization - Gert Weber (in Room "Kino") Practical Part D -> Data processing using XDS and XDSAPP - Manfred Weiss (in Room "Kino") |
| 17:30 | Discussion |
Wednesday, 12th October 2022HZB, Room "Kino", Magnusstr. 2, 12489 Berlin |
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| 9:00 | Fragment-Screening and HT crystallography - Jan Wollenhaupt (HZB) |
| 10:00 | Model validation in MX - Tobias Bock-Bierbaum (MDC) |
| 11:00 | Coffee Break |
| 11:30 | Micro electron diffraction in MX - Hongyi Xu (University Stockholm) |
| 12:30 | Lunch Break |
| 13:30 |
Moving everybody to MDC Campus Berlin-Buch, Building 83 (MDC.C), Room Dendrit 2 & 3 Robert-Rössle-Str. 10, 13125 Berlin |
| 15:30 | Integrative structural biology on the centrosome - Andrea Musacchio (MPI Dortmund) |
| 16:30 | Developing Solid State NMR as a Versatile Tool for Integrated Structural Biology - Adam Lange (FMP Berlin) |
| 17:30 | Dinner and poster session |
Thursday, 13th October 2022MDC Campus Berlin-Buch, Building 83 (MDC.C), Room Dendrit 2 & 3, Robert-Rössle-Str. 10, 13125 Berlin |
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| 9:30 | Introduction to NMR - Markus Zweckstetter (MPI Göttingen, DZNE) |
| 10:30 | Drug screening with NMR - Michael Sattler (HMGU, Munich) |
| 11:30 | Lunch Break |
| 12:30 | NMR practical exercises - Peter Schmieder (FMP Berlin) |
| 16:00 | Studying dynamics in large assemblies and a view on including Alphafold in NMR projects - Hartmut Oschkinat (FMP Berlin) |
| 18:30 | Social Dinner in the City and Poster prize |
Friday, 14th October 2022MDC Campus Berlin-Buch, Building 83 (MDC.C), Room Dendrit 2 & 3, Robert-Rössle-Str. 10, 13125 Berlin |
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| 9:00 | Introduction into CryoEM - Carsten Sachse (FZ Jülich) |
| 10:00 | Single particle CryoEM - Christoph Diebolder (Charité) & Thiemo Sprink (MDC) |
| 11:00 | Cryo electron tomography approaches - Misha Kudryashev (MDC) |
| 12:00 | Lunch Break |
| 12:45 | Practical exercises for Single particle and CryoET / CLEM in five groups |
| 15:30 | Final discussion |
| 16:00 | Departure |