Development and Applications of X-ray Microscopy for Biology

Dr. James McNally has worked at the interface of biology and physics throughout his career. His experience includes the construction and development of a deconvolution microscope in the 1990’s with application to various problems in live cell microscopy, including the analysis of cell motion within a tissue mass. In the 2000’s he developed several quantitative methods for the in vivo measurement of transcription factor binding to chromatin, including approaches using fluorescence recovery after photobleaching, fluorescence correlation spectroscopy and single molecule tracking, thereby establishing a gold standard for in vivo binding measurements. His most recent interests focus on the development of X-ray microscopy as a tool for 3D ultrastructural imaging of intact biological specimens.  For his publications, please see here.