Roohani, S.; Loskutov, J.; Heufelder, J.; Ehret, F.; Wedeken, L.; Regenbrecht, M.; Sauer, R.; Zips, D.; Denker, A.; Joussen, A.M.; Regenbrecht, C.R.A.; Kaul, D.: Photon and Proton irradiation in Patient-derived, Three-Dimensional Soft Tissue Sarcoma Models. BMC Cancer 23 (2023), p. 577/1-10
10.1186/s12885-023-11013-y
Open Access Version
Abstract:
Despite their heterogeneity, the current standard preoperative radiotherapy regimen for localized high-grade soft tissue sarcoma (STS) follows a one fits all approach for all STS subtypes. Sarcoma patient-derived three-dimensional cell culture models represent an innovative tool to overcome challenges in clinical research enabling reproducible subtype-specific research on STS. In this pilot study, we present our methodology and preliminary results using STS patient-derived 3D cell cultures that were exposed to different doses of photon and proton radiation. Our aim was: (i) to establish a reproducible method for irradiation of STS patient-derived 3D cell cultures and (ii) to explore the differences in tumor cell viability of two different STS subtypes exposed to increasing doses of photon and proton radiation at different time points.