HZB and Freie Universität Berlin establish the joint research group “X-Ray Microscopy” for studying complex cellular processes
In May this year, the joint research group “X-Ray Microscopy” was launched, combining the expertise of teams led by Prof. Dr. Gerd Schneider (Helmholtz-Zentrum Berlin) and Prof. Dr. Helge Ewers (Freie Universität Berlin). While Ewers’ group contributes its experience in the field of optical microscopy and biological research, the HZB workgroup is responsible for X-ray microscopy at the synchrotron source BESSY II. The two methods help researchers to gain a detailed insight into the processes taking place inside cells.
We are very pleased about the new cooperation with Prof. Ewers’ workgroup. It gives our own activities in this field a much stronger connection to the biological research being done at the university,” says Prof. Dr. Gerd Schneider. The core duties of his department at HZB include making advancements to the x-ray microscopes and lenses at the synchrotron source BESSY II. The active exchange between the new cooperation partners will give a new boost to method development, says Schneider. Prof. Dr. Helge Ewers is also excited about the future-oriented cooperation: “X-ray microscopy opens up entirely new possibilities for us in the research of intracellular processes.”
The joint research group is all about the complementary use of optical and X-ray microscopy. Optical microscopy and super-resolution methods are excellent for locating proteins marked with dye molecules in tissue samples. X-ray microscopy, in turn, allows correlative imaging of the distribution of proteins, viruses or nanoparticles over a relatively large section in high-resolution and three-dimensions. The two microscopy methods thus deliver a comprehensive picture of the intracellular structures and processes.
After a successful upgrade, the X-ray microscope TXM at the synchrotron source BESSY II is now available again to users. Aside from biological studies, which can now be conducted with the combined expertise in the joint research group, the X-ray microscope is used above all for exploring various questions of materials and energy research.
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- Protein crystallography at BESSY II: faster, better and more and more automaticMany diseases are linked to malfunctions of proteins in the organism. The three-dimensional architecture of these molecules is often highly complex, but it can provide valuable insights into biological processes and the development of drugs. X-ray diffraction at the MX beamlines of BESSY II can be used to decipher the 3D structure of proteins. To date, more than 5000 structures have been solved at the three MX beamlines. Here, we present a review and an outlook with Manfred Weiss, head of the research group for macromolecular crystallography.
- 5000th protein structure at BESSY II: Starting point for a COVID drugMany proteins have a complex architecture that enables biological functions. Molecules can bind to specific sites on a protein and alter its function. A team at HZB has now investigated the Nsp1 protein, which plays a role in infection with the SARS-CoV-2 virus. They analysed protein crystals, previously mixed with molecules from a fragment library, and discovered a total of 21 candidates as starting points for drug development. At the same time, they also decoded the 5000th structure at BESSY II.