Lautner, G.; Kaev, J.; Reut, J.; Öpik, A.; Rappich, J.; Syritski, V.; Gyurcsányi, R.E.: Selective artificial receptors based on micropatterned surface-imprinted polymers for label-free detection of proteins by SPR imaging. Advanced Functional Materials 21 (2011), p. 591-597
10.1002/adfm.201001753
Abstract:
Here we introduce a novel concept to generate micropatterned surface-imprinted polymers (SIPs) for protein recognition by using standard photolithographic technology. Avidin- imprinted PEDOT/PSS conducting polymer microbands are prepared directly on SPR chips, which enable convenient and label-free monitoring of the binding events. The novel surface-imprinted microstructures bind the template protein, avidin, used as a model with dissociation constants in the submicromolar range (125 nM). The SIPs have an avidin-binding capacity about one order of magnitude higher than the corresponding nonimprinted polymers and are capable of discriminating among functional homologues of avidin, i.e., neutravidin, extravidin, and streptavidin.