New detector accelerates protein crystallography

60s on the new detector were sufficient to obtain the electron density of the PETase enzyme.

60s on the new detector were sufficient to obtain the electron density of the PETase enzyme. © HZB

<p class="MsoPlainText">The MX-beamline 14.1 has been upgraded with a new, better, faster and more sensitive PILATUS-detector.</p> <p class="MsoPlainText">&nbsp;

The MX-beamline 14.1 has been upgraded with a new, better, faster and more sensitive PILATUS-detector.

  © HZB

Last week a new detector was installed at one of the three MX beamlines at HZB. Compared to the old detector the new one is better, faster and more sensitive. It allows to acquire complete data sets of complex proteins within a very short time.

Proteins consist of thousands of building blocks that can form complex architectures with folded or entangled regions. However, their shape plays a decisive role in the function of the protein in the organism. Using macromolecular crystallography at BESSY II, it is possible to decipher the architecture of protein molecules. For this purpose, tiny protein crystals are irradiated with X-ray light from the synchrotron source BESSY II. From the obtained diffraction patterns, the morphology of the molecules can be calculated.

Now the MX team at BESSY II has put a new detector into operation at the MX beamline 14.1, which works about 2 to 3 times faster than before. The team analysed a crystal from the enzyme PETase as a sample. PETase does partially degrade the plastic PET. In less than a minute, the detector was able to record a complete diffraction data set, which includes data from an angular range of 180 degrees. The data set consists of 1200 images, each of which was exposed to X-rays for 45 milliseconds. "The resulting electron density was of excellent quality and showed all structural features of the enzyme," explains Dr. Manfred Weiss, who leads the MX team at BESSY II.

The success of the HZB MX beamlines is documented by more than 3000 PDB entries from experimental beamtime from more than a hundred international user groups from academia and pharmaceutical research companies.

red.

You might also be interested in

  • 8th World Conference on PV Energy Conversion
    News
    19.09.2022
    8th World Conference on PV Energy Conversion
    The WCPEC-8 woll take place from 26 – 30 September 2022 in the Milano Convention Centre in Milan, Italy.
    Also scientists from PVcomB will present latest results about their research work to photovoltaics.

  • 40 years of research with synchrotron light in Berlin
    News
    14.09.2022
    40 years of research with synchrotron light in Berlin
    Press release _ Berlin, 14 September: For decades, science in Berlin has been an important driver of innovation and progress. Creative, talented people from all over the world come together here and develop new ideas from which we all benefit as a society. Many discoveries – from fundamental insights to marketable products – are made by doing research with synchrotron light. Researchers have had access to this intense light in Berlin for 40 years. It inspires many scientific disciplines and is an advantage for Germany.

  • New road towards spin-polarised currents
    Science Highlight
    08.09.2022
    New road towards spin-polarised currents
    The transition metal dichalcogenide (TMD) series are a family of promising candidate materials for spintronics. A study at lightsource BESSY II has unveiled that in one of those materials even simple linear polarised light is sufficient to selectively manipulate spins of different orientations. This result provides an entirely new route for the generation of spin-polarised currents and is a milestone for the development of spintronic and opto-spintronic devices.